Download Advanced Fluorescence Microscopy: Methods and Protocols by Peter J. Verveer PDF

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By Peter J. Verveer

This quantity presents an outline of complex fluorescence microscopy, overlaying a huge diversity of equipment. every one bankruptcy makes a speciality of a unique procedure and offers a realistic advisor for software in organic platforms. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and pointers on troubleshooting and keeping off identified pitfalls.

Authoritative and state-of-the-art, Advanced Fluorescence Microscopy: tools and Protocols seeks to supply scientists with equipment for organic structures which are of curiosity.

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Weisswange I, Bretschneider T, Anderson KI (2005) The leading edge is a lipid diffusion barrier. Millán J, Hewlett L, Glyn M et al (2006) Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola-and F-actin-rich domains. Saffarian S, Kirchhausen T (2008) Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane. Pitkeathly WT, Poulter NS, Claridge E et al (2011) Auto-align – multi-modality fluorescence microscopy image co-registration.

Sund SE, Swanson JA, Axelrod D (1999) Cell membrane orientation visualized by polarized total internal reflection fluorescence. Axelrod D (1981) Cell-substrate contacts illuminated by total internal reflection fluorescence. Stock K, Sailer R, Strauss W et al (2003) Variable‐angle total internal reflection fluorescence microscopy (VA‐TIRFM): realization and application of a compact illumination device. Fish KN (2009) Total internal reflection fluorescence (TIRF) microscopy. Olveczky BP, Periasamy N, Verkman A (1997) Mapping fluorophore distributions in three dimensions by quantitative multiple angle-total internal reflection fluorescence microscopy.

Golshani P, Portera-Cailliau C (2008) In vivo 2-photon calcium imaging in layer 2/3 of mice. MacLean J, Yuste R (2005) Imaging action potentials with calcium indicators: practical guide. In: Yuste R, Konnerth A (eds) Imaging neurons a laboratory manual, 2nd edn. Feng G, Mellor RH, Bernstein M et al (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Grutzendler J, Kasthuri N, Gan WB (2002) Long-term dendritic spine stability in the adult cortex. Trachtenberg JT, Chen BE, Knott GW et al (2002) Long-term in vivo imaging of experience-­ dependent synaptic plasticity in adult cortex.

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